Analysis of anti-enterococcal activity of three honey samples in golestan province

Understanding the resistance patterns of bacteria and their sensitivity and attempting to find new compounds with broad effective spectrum would be significant in controlling infection. This study aimed at evaluating antibacterial potential of three honey samples produced in Golestan province, Iran, against Enterococcus faecalis strains. After the isolation and identification of bacterial strains, their antibiotic resistance was determined using Kirby-Bauer method. Then, seven isolates of Enterococcus faecalis with multidrug-resistance were selected and antibacterial activities of honey samples assessed by disk-diffusion, well-diffusion and minimum inhibitory concentration test [MIC]. The highest diameter of inhibition zone in disk-diffusion and well diffusion methods is 20 mm and 26 mm, respectively. Also the MIC is measured 62.5 mg.ml for all samples, In prohibiting microbial growth, all three samples were successful and they could prevent growing bacterial infection in spite of remarkable resistance of Entrococcus. Hence, further research should be conducted to assess the effects of honey samples against other bacteria

Isolation, molecular identification and evaluation of antifungal effects of the bacilli isolated from rhizospheric soil of gorgan region against tricopfifton mentegrqp aytes

Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The isolates that had the highest antifungal effects were analyzed by PCR and 16 srRNA sequencing. Of 54 strains, 14 have antifungal activity. The Isolates, S4 and SI2, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% honiology with Bacillus cereusstrain KU4 and Bacillus thurmgienslsstrain ucsc 27. According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes